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Plankton Ecology
The Plankton Ecology Area is located at Naples centre. The Area allows users to analyze and study phytoplankton and zooplankton, from receiving sea samples, to isolating and culturing the species of interest, to allow their physiological study, population and genetics.
The area is temporarily distributed, pending the completion of the renovation works of the west wing, in 7 rooms and 2 common areas distributed on 4 of the 5 floors of the East wing, as listed below according to the activities carried out:
Wet Lab - Ground floor room #T35
Maintenance of phytoplankton and zooplankton cultures: First floor room #148 and third floor WALK IN cell #365ter
Live zooplankton manipulations - First floor room #138
Live phytoplankton manipulations – First floor rooms #138 and #148 and Third floor room #364
Fixed Phyto- and ZooPlankton manipulations - Third floor rooms #325 and #326
GROWTH CHAMBERS for EXPERIMENTS: floor -1 (along the wall in front of the stairs) and third floor (hall #365)
Functional Area Manager: Dott.ssa Carmen Minucci This email address is being protected from spambots. You need JavaScript enabled to view it. Internal Phone Number #272 |
Bookings on www.labagenda.com
Molecular Ecology
The Molecular Ecology Area is devoted to investigated molecular aspects related to taxonomic identification, population genetics studies, gene expression studies and functional genetics.
The area is temporarily distributed, pending the completion of the renovation works of the west wing in the following rooms:
- East wing, first floor rooms #140 and #150
- East wing, first floor room #146
- East wing, third floor room #340
- East wing, third floor room #346
Functional Area Manager Camilla Borgonuovo This email address is being protected from spambots. You need JavaScript enabled to view it. |
Bookings on www.labagenda.com
Biochemistry and Cell Biology
The Biochemistry and Cell Biology area is equipped with various instruments useful for the biochemical and functional characterization of proteins and small molecules, including the study of signal transduction in marine organisms. The available equipment includes homogenizers for cell destruction, chromatographic systems for the purification and characterization of proteins, spectrophotometers for enzymatic assays.
Manager: Imma Castellano
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Extension #293
Third floor EST Wing, #356
Bookable instruments |
- Chromatographic System/FPLC Acta |
- Spectrophotometer Agilent |
- Spectrophotometer Agilent Cary 100 Scan |
- Homogenizer ultraturrax Junke&Kunkel |
- Incubator Thermo scientific |
Functional Genomics
The area of Functional Genomics is devoted to the localization and perturbation of gene expression during embryonic development in marine organisms. This area is organized into dedicated two platforms:
Manager: Mara Francone
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Extension #353
Second floor EAST Wing
Gene perturbation
The Gene Perturbation platform is equipped with the necessary equipment for the transformation and culture of embryos of marine organisms.
The area is equipped with: a puller to prepare microinjection needles, an electroporator and three microinjection setups necessary for the transformation of zygotes.
Refrigerated incubators and a thermostatic chamber (walk-in) are also available, for the growth of embryos at the required temperature (typically 15, 18 and 20°C).
Managere: Mara Francone
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Extension #353
Second floor EAST Wing, #244, #246
|
- Electroporator |
- Puller |
- Microinjection setup 1 |
- Microinjection setup 2 |
- Microinjection setup 3 |
- Panasonic incubator (15°C) |
- Memmert incubator (18°C) |
- VelpScientifica incubator (20°C) |
- Walk-in room (18°C) |
Book on LabAgenda: instructions for users |
Microscopic observations
The Microscopic Observations platform is equipped with four systems:
2 microscopes, both with visible light and fluorescence;
2 stereomicroscopes (brightfield and fluorescence).
Manager: Mara Francone
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Extension #353
Second floor EAST Wing, #245
Bookable instruments |
Zeiss Apotome.2 microscope |
Zeiss Imager.M1 microscope |
Leica M205FA stereomicroscope |
Leica M205C stereomicroscope |
Book on LabAgenda: instructions for users |
Physiology and Behavior
The Physiology and Behavior area is devoted to research in fields including physiology, neuroscience and behavior of marine organisms, with a focus on the evaluation of neural and behavioral plasticity. This is organized into three dedicated platforms:
Manager: Pamela Imperadore
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Extension #281
First Floor EAST Wing
Developmental Molecular Biology
The Developmental Molecular Biology area is dedicated to the analysis and study of the regulatory mechanisms that govern development of marine organisms. This includes the following dedicated platforms:
- Electrophoresis and PCR
- Centrifugation and Ultracentrifugation
- Nucleic Acid Extraction and Purification
Manager: Alessandro Amoroso
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Extension #345
First Floor EST Wing | Second Floor EST Wing |
Electrophoresis and PCR
The Electrophoresis and PCR platform is equipped with several PCR machines (9) and with the necessary equipment for the: preparation of agarose gel, electrophoresis gel running, UV image acquisition, quantification of nucleic acids (NanoDrop).
Managere: Alessandro Amoroso
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Extension #345
Bookable Equipment | |
Room #146 | |
Chemical Hood for the purification of nucleic acids | |
Room #235 | |
- PCR HYBAID Express #1 | |
- PCR HYBAID Express #2 | |
- PCR HYBAID PX2 | |
- PCR BIORAD C1000 #1 | |
- PCR BIORAD C1000 #2 | |
- PCR MJResearch PTC 100 | |
- PCR MJResearch MiniCycler | |
- PCR GeneAmp System 9700 | |
- PCR EPPENDORF Mastercycler | |
- Thermostated Bath JULABO SW22 | |
- Thermostated Bath GFL | |
- Hybridization Oven FINEPCR | |
- Hybridization Oven TECNE | |
Book on LabAgenda: instructions for users |
Primo piano Ala EST, #146 |
Secondo piano Ala EST, #235 |
Centrifugation and Ultracentrifugation
The Centrifugation and Ultracentrifugation platform is equipped with various instruments which allow centrifugation at different speeds (up to 90000 rpm in the case of Ultracentrifugation), with fixed angle or swinging bucket rotors, at controlled temperature.
Manager: Alessandro Amoroso
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Extension #345
Bookable Equipment |
Room #246 |
- Concentrator EPPENDORF 5301 |
- Refrigerate Centrifuge 15/50mL EPPENDORF 5810R |
- Refrigerate Centrifuge EPPENDORF 5430R |
- Incubator Shaker INNOVA 4 |
Landing |
- Ultracentrifuge BECKMAN Optima XL 100K |
- Centrifuge BECKMAN AvantiJ25 |
- Centrifuge BECKMAN J2 MC |
Second Floor Room #246 and landing |