The Sequencing and Molecular Analysis Center (CSAM) offers services, training and consultancy in the Molecular Biology field, from the execution of complex procedures to the development of on-demand protocols. This Core Facility developed a sequencing service which includes both classic Sanger with the 3730xl DNA Analyzer, and deep sequencing with the Ion GeneStudio S5, a Real Time PCR and a droplet digital PCR services with the Thermo ViiA7 and QuantStudio 5, and Biorad Qx200 ddPCR instruments, respectively. The Covaris M220 ultrasonicator allows an effective DNA fragmentation for NGS sequencing of Chromatin Immunoprecipitation experiments, for epigenetic studies. The Tecan Freedom EVO 200 liquid handler is devoted to the set-up of high throughput protocols for nucleic acids purification and preparation of real time PCR , ELISA or other assays with a high number of samples in the 96 or 384 well formats. CSAM also supplies the Intitute weekly with oligonucleotides, competent cells, solid and liquid bacterial culture media. Services and instruments available can be booked on Labarchives, for other request feel free to contact us at This email address is being protected from spambots. You need JavaScript enabled to view it.

Responsabile

Pasquale De Luca

Tel. +39 081 5833453
Fax: +39 081 7641355
e-mail pasquale.deluca(at)szn.it

The Labs of the Advanced Micrsocopy Center (CMA) host at present two spectral confocal microscopies, an epifluorescence microscope equipped with a thermostatic chamber with CO2 control for live cell imaging, a transmission electron microscope two scanning electron microscopes and all the necessary instruments for sample preparation for both the classic chemical fixation and the crio-fixation.
The personnel of the Center is highly specialised, to ensure the correct application of optic and electron microscopy and to give internal and external users scientific consultancy, technical assistance and formation. Moreover, a section dedicated to research, which strictly interacts with the technical personnel, provides the development of new applications and protocols, especially in the field of living cells and organisms.
Microscopes and preparative instruments are accessible to users following one of these ways:
1) independent use (after education);
2) assisted use, with our personnel supervision;
3) as a service, entirely or partially demanded to our personnel, conveniently informed on the goals of the observations and, possibly, assisted by the researcher during the image acquisition (strongly recommended).

Responsible

Fabio Formiggini PhD
Tel. +39 081 19933100
e-mail: fabioformiggini.szn(at)gmail.com

From 2017 the activities of the new structure have begun with the support and coordination of the Prof. Maria Luisa Chiusano, Professor of Molecular Biology and Bioinformatics at Federico II. The core facility currently includes a researcher with expertise in marine metagenomics, a technician with expertise as IT system and infrastructer engineer, a technician with expertise in genomics, transcriptomics and proteomics and a research assignee with expertise in georeferenced data analysis and geographic informatic systems (GIS). The unit is dedicated to the setting up of advanced computing systems for the analysis of "omics" data and to the implementation of methodologies, software, IT procedures and databases, maintenance and release of data produced by the institute. The unit is also dedicated to training activities supporting marine research for the Institute.

Responsabile

Maria Luisa Chiusano

+39 0815833452

bioinforma(at)szn.it

chiusano(at)unina.it

The section "Access to marine ecosystems and environmental analysis" consists of the Units IRM, IMOM, MAA and ITR, in a multidisciplinarity context created by the interaction between the different units. Thanks to the contribution of the research and infrastructure projects PON PRIMA, PON InSEA, PON PLaCE, and others forthcoming, the Section will be equipped with high technological value instrumentation and multidisciplinary fixed observatories both coastal and deep that are fully part of the European and international context for ecosystem monitoring services, for the preservation of the state of environmental quality, needed for preserving and regenerate natural capital (see e.g. EMSOERIC, EMBRC-ERIC, CLUSTER-BIG). This is in consideration of the fact that marine resources will be in the near future the object of greater use by human activities. The expansion of marine research must interact strongly with the many economic activities that take place at sea and eco-sustainable exploitation of its natural resources. In this scenario the role of the Stazione Zoologica and in particular of the Section for Access to Marine Ecosystems and Environmental Analysis of the RIMAR Department, is preeminent and it will therefore be necessary to focus on technological development thanks to the strong interaction of the connection between Core facilities and Infrastructure for research at sea (IRM), Implementation, Technology & Robotics (ITR) and Environmental Monitoring and Analysis (MAA).

Coordinator

Simonepietro Canese

Stazione Zoologica Anton Dohrn
Via Po' 25c
00189 Roma - Italia

Tel.: +39 06 21710215
Mobile: +39 3356790469
E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Contatto Skype: simonepietro.canese