The Molecular Ecology Area is devoted to investigated molecular aspects related to taxonomic identification, population genetics studies, gene expression studies and functional genetics.

The area is temporarily distributed, pending the completion of the renovation works of the west wing in the following rooms:

• East wing, first floor rooms #140 and #150
• East wing, first floor room #146 
• East wing, third floor room #340
• East wing, third floor room #346

Functional Area Manager Camilla Borgonuovo This email address is being protected from spambots. You need JavaScript enabled to view it.  Room #140 Internal Phone Number #236

Bookings on www.labagenda.com

The Biochemistry and Cell Biology area is equipped with various instruments useful for the biochemical and functional characterization of proteins and small molecules, including the study of signal transduction in marine organisms. The available equipment includes homogenizers for cell destruction, chromatographic systems for the purification and characterization of proteins, spectrophotometers for enzymatic assays.

Manager: Imma Castellano

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Extension #293

Third floor EST Wing, #356

Book on LabAgenda: instructions for users

The area of Functional Genomics is devoted to the localization and perturbation of gene expression during embryonic development in marine organisms. This area is organized into dedicated two platforms:

• Gene perturbation
• Microscopic observations

Manager: Mara Francone
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Extension #353

Second floor EAST Wing

The Gene Perturbation platform is equipped with the necessary equipment for the transformation and culture of embryos of marine organisms.
The area is equipped with: a puller to prepare microinjection needles, an electroporator and three microinjection setups necessary for the transformation of zygotes.
Refrigerated incubators and a thermostatic chamber (walk-in) are also available, for the growth of embryos at the required temperature (typically 15, 18 and 20°C).  

Managere: Mara Francone
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Extension #353

Second floor EAST Wing, #244,  #246 

The Microscopic Observations platform is equipped with four systems: 
2 microscopes, both with visible light and fluorescence; 
2 stereomicroscopes (brightfield and fluorescence).

Manager: Mara Francone
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Extension #353

Second floor EAST Wing, #245

The Physiology and Behavior area is devoted to research in fields including physiology, neuroscience and behavior of marine organisms, with a focus on the evaluation of neural and behavioral plasticity. This is organized into three dedicated platforms:

• Histology
• Behavioral and morphological analysis
• Electrophysiology

Manager: Pamela Imperadore 

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Extension #232

First Floor EAST Wing

The Developmental Molecular Biology area is dedicated to the analysis and study of the regulatory mechanisms that govern development of marine organisms. This includes the following dedicated platforms:

• Electrophoresis and PCR
• Centrifugation and Ultracentrifugation
• Nucleic Acid Extraction and Purification

Manager: Alessandro Amoroso

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Extension #345

First Floor EST Wing	Second Floor EST Wing

The Electrophoresis and PCR platform is equipped with several PCR machines (9) and with the necessary equipment for the: preparation of agarose gel, electrophoresis gel running, UV image acquisition, quantification of nucleic acids (NanoDrop).

Managere: Alessandro Amoroso

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Extension #345

Bookable Equipment
Room #146
Chemical Hood for the purification of nucleic acids
Room #235
- PCR HYBAID Express #1
- PCR HYBAID Express #2
- PCR HYBAID PX2
- PCR BIORAD C1000 #1
- PCR BIORAD C1000 #2
- PCR MJResearch PTC 100
- PCR MJResearch MiniCycler
- PCR GeneAmp System 9700
- PCR EPPENDORF Mastercycler
- Thermostated Bath JULABO SW22
- Thermostated Bath GFL
- Hybridization Oven FINEPCR
- Hybridization Oven TECNE
Book on LabAgenda: instructions for users

Primo piano Ala EST, #146	Secondo piano Ala EST, #235

The Centrifugation and Ultracentrifugation platform is equipped with various instruments which allow centrifugation at different speeds (up to 90000 rpm in the case of Ultracentrifugation), with fixed angle or swinging bucket rotors, at controlled temperature.

Manager: Alessandro Amoroso

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Extension #345

Second Floor Room #246 and landing

Book on LabAgenda: instructions for users

The Nucleic Acid Extraction and Purification platform is organized in different locations: one on the first floor and two on second floor of the East Wing. These laboratories are equipped with hoods dedicated to various activities.

Manager: Alessandro Amoroso

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Extension #345

Bookable Equipment
Room #146
Chemical Hood for the purification of nucleic acids
Room #242
Chemical Hood equipped with  pHmeter THERMO SCIENTIFIC Orion Star A211 for the preparation of solutions
Room #238
Chemical Hood for the purification of nucleic acids
Book on LabAgenda: instructions for users

Primo piano Ala EST, #146	Secondo Piano Ala EST: Stanze # 242 e 238