The Sequencing and Molecular Analysis Center (CSAM) offers services, training and consultancy in the Molecular Biology field, from the execution of complex procedures to the
development of on-demand protocols. This Core Facility developed a sequencing service which includes both classic Sanger with the 3730xl DNA Analyzer, and deep sequencing with the Ion GeneStudio S5, a Real Time PCR and a droplet digital PCR services with the Thermo ViiA7 and QuantStudio 5, and Biorad Qx200 ddPCR instruments, respectively. The Covaris M220 ultrasonicator allows an effective DNA fragmentation for NGS sequencing of Chromatin Immunoprecipitation experiments, for epigenetic studies. The Tecan Freedom EVO 200 liquid handler is devoted to the set-up of high throughput protocols for nucleic acids purification and preparation of real time PCR , ELISA or other assays with a high number of samples in the 96 or 384 well formats. CSAM also supplies the Intitute weekly with oligonucleotides, competent cells, solid and liquid bacterial culture media. Services and instruments available can be booked on Labarchives, for other request feel free to contact us at sma.center@szn.it
Responsabile
Tel. +39 081 5833453
Fax: +39 081 7641355
e-mail pasquale.deluca(at)szn.it
