Reference: Maria I. Arnone
Laboratory dedicated to the study of embryonic development of marine organisms, including functional analysis of gene regulatory networks.
It includes areas for molecular biology and in situ hybridization, microinjection and electroporation of zygotes.
It also includes classic and basic biological laboratory equipments.
Systems for experiments
DevBio Lab uses different model organisms such as ascidians (Ciona, Molgula), sea urchins (Paracentrotus, Strongylocentrotus), sea stars (Patiria), amfioxus (Branchistoma) and fishes (Danio).
The lab carries it activities in collaboration with units Meda e MaRe for collecting, rearing and keeping these organisms.
Analysis
- Spatial and temporal study of gene expression during embryonic development by in situ hybridization, immunohistochemistry, Real Time qPCR (in collaboration with Mb&Bi unit), western blots.
- Disruption of gene expression (knock-out, knock-down, overexpression and transgenesis) by microinjection (antisense oligonucleotides, RNA) or elecroporation (DNA).
- gene expression and morphofunctional analysis fenotipi ottenuti.
Equipments
- Systems for microinjection (microscopes, including stereo and inverted microscopes) in sea urchins, sea squirts and fish.
- Electroporation in eukaryotic cells.
- Stereomicroscopes for observation and manipulation of embryos.
- Cells thermostated (at various temperatures) for the culture of embryos and larvae.
- Microscopes equipped with high-resolution cameras for spatial analysis of gene expression.
- Analysis of movement to characterize behavioral phenotypes.
Reference: Filomena Ristoratore
Laboratory equipped for the study of molecular aspects of physiological responses of marine organisms at various stages of life cycle.
Includes areas equipped for electrophysiology and molecular biology, cell culture and basic laboratory equipments.
Systems for experimentation
- Genomic amplification and gene expression.
- Electrophysiology.
Analysis
- Physiological responses of marine organisms to stimuli.
- Membrane potential during fertilization events of marine organisms.
- Electrophysiology and Neurophysiology.
- Physiological analysis of immune responses in marine invertebrates.
Equipments
- Eletrophysiological Set-ups.
- Cell culture apparata.
- Molecular biology (nucleic acids and proteins).
Reference: Maria Costantini
The Laboratory of Marine Genomics and Epigenetics is aimed at developing novel approaches for the study of genomes of marine organisms by applying multidisciplinaty approaches.
It encompasses approaches for the study of epigenetic modifications to study the evolution of diversity and adaptative capabilities of organisms.
The Lab includes areas equipped for molecular biology and genomic and proteomic analysis.
Systems for experimentation
- Facilities for molecular analysis applied to the study of genomes of marine organisms.
- Systems for the morpho-functional characterization and genome analysis.
Analysis
- Genomic approaches to study genome organization in marine organisms.
- Transcriptomics.
- Genomic analysis of the functioning of marine organisms and systems.
- Gene expression (Real Time qPCR, Western Blot).
- Proteomic approaches to study epigenetic modifications (metilation, de-metilation, acetylation, histone and chromatine modifications, etc.).
- Proteomic approaches (antibodies) to identify epigenetic modifications induced by evolution and interaction of organisms with the environment.
Equipments
- Electrophoresis, DNA and proteins.
- PCRs including gradient.
- Scanners for micro-arrays and gene expression.
Reference: Remo Sanges
Laboratory equipped for studies of computational genomics throughout bioinformatics approaches.
Systems for experimentation
Databases, high throughput functional genomics.
Analysis
Computational approaches, bioinformatics, comparative genomics, data-mining (genomics and transcriptomics in marine organisms).
Strumentazioni
- Personal computers.
- Workstations and servers.
The Ischia Marine Center (IMC) is an interdepartmental center located on the Island of Ischia., The research focus of the IMC is on vulnerability assessment of marine coastal ecosystems and organisms through an integrated approach, highlighting the interactions between anthropogenic activities and climate change, with applications to environmental protection, outreach, and restoration actions. The aim of IMC research activities is to facilitate cooperative research between internal and external researchers, interdisciplinary collaborations, and interactions with public and private stakeholders.
IMC main lines of research include:
1) Benthos ecology: the study of the effects of global changes on biodiversity, plant-animal interaction, and the microbiome associated with marine species.
2) Ecophysiology and biotechnology: the morpho-functional study of coastal communities associated with seagrasses and chemical ecology, also through cultivationof model organisms and research of new biotechnologies.
3) Fisheries oceanography: the study of fish eggs and larvae to understand the variations in abundance and distribution of fish populations related to regional oceanographic factors and global change in climate.
4) Taxonomy of marine organisms: the Marine Organisms Taxonomy Unit (MOTax) at the IMC focuses on the analysis of the benthic communities (sorting, LPT identification, analysis of ecological data).
The IMC is organized into three structures with two functional areas:
Villa Dohrn, historical site, built in 1906 as the private residence of the Dohrn family. In 1969 the building became the Benthos Ecology Laboratory. Villa Dohrn is currently undergoing a major renovation and repurposing project, to become the new "Research Center for Adaptation of Marine Ecosystems to Climate Change".
MEDAS Center (Marine Ecological Data Analysis and Synthesis), with the aim of analyzing existing information to address the pressing questions and needs, including economic ones, in the context of Blue Growth. At the momentMEDAS is the operational headquarters of the IMC and the Ecology Functional Area of the Benthos/Nekton for the entire EMI Department of the SZN.
Diving Centre, that oversees scientific diving to support research activities at sea, with Functional Area Diving Center Ischia, located near the mooring of the SZN vessel Beagle Junior, operated by the IMC.
CoordinatorMaria Cristina Vigo Majello |
![]() |
Personnel
Coordinator
Maria Cristina Vigo Majello
Administration
Messina Rosanna
Patti Francesco
Senior Researcher
Ciannelli Lorenzo
Teixidó Núria
Zupo Valerio
Researcher
Mazzella Valerio
Technologist
Lorenti Maurizio
Technician
Chiarore Antonia
Guglielmo Rosanna
Iacono Bruno
Mirasole Alice
Rando Vincenzo
Scholarship
Italiano Anna
PhD student
Liguori Gianluca
Nannini Matteo
Somma Emanuele










