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SECRETED - Sustainable Exploitation of bio-based Compounds Revealed and Engineered from naTural sources

SEDREMED - Bioremediation of contaminated sediments in coastal areas of ex-industrial sites 2021-2024

ISIDORE - Integrated Services For Infectious Diseases Outbreak Research

HOTBIO - A holistic approach to training a new generation of scientists in marine biodiscovery

BlueRemediomics - ARNESSING THE MARINE MICROBIOME FOR NOVEL SUSTAINABLE BIOGENICS AND ECOSYSTEM SERVICES

Project @ CNR “SCANFISH” SEA COSMETICS AND NUTRACEUTICS: FROM FISHERY AND AQUACULTURE BYPRODUCTS

PON project “ALIFUN” Development of functional foods for the innovation of traditional Italian food products

PRoDiA: Droplet digital PCR to assess the occurrence and infer the ROle of prostaglandin in natural DIatom Assemblage in the Ionian coastal waters

ABBaCo - Restauro Ambientale e Balneabilità del SIN Bagnoli-Coroglio

DIsCO - Diatom life cycles, molecular controls and contribution to ecosystem dynamics

EMSO MedIT - PON R&C 2007-2013 - PAC Potenziamento infrastrutture di ricerca pubblica - Linea di intervento B. Interventi per l’adeguamento e il consolidamento di infrastrutture per il monitoraggio ambientale

ESSEM COST Action ES0906 - Seagrass productivity: from genes to ecosystem management

GRASSMET - Climate change effects on seagrass secondary metabolism: ecological implications

HighGrass - High-CO2 effects on seagrass photosynthetic ecophysiology

INBALANCE - INvertebrate-BActeriaL Associations as hotspots of benthic Nitrogen Cycling in Estuarine ecosystems

IRMA - Implementazione e connessione Remota di una piattaforma per il monitoraggio in tempo reale di microorganismi MArini Finanziato con Decreto MIUR del 26-3-2014

OCEAN MEDICINES - Marie Skodowska-Curie Research and Innovation Staff Exchange (RISE) finanzia partners accademici e industriali per sviluppare composti da microorganismi marini con attività anticancerogena e anti-infettiva

PALEOPARK - Seagrass palaeo-records as a tool for the evaluation, diagnosis and prognosis of the evolution of species, communities, and processes in Spanish insular National Parks

PharmaDeep - Progetto Europeo EUROFLEET2 che finanzia una campagna oceanografica nei mari subantartici per campionare organismi a grande profondità (5000m) per potenziali applicazioni in campo biomedico

PharmaSea - Progetto Europeo focalizzato sulla ricerca, sviluppo e commercializzazione di nuove biomolecole da organismi marini

PON01_00117 Antigeni e Adiuvanti per Vaccini e Immunoterapia.
Capofila: Novartis Vaccines & Diagnostics srl

PON01_02093 Studio di nuove tecnologie e piattaforme tecnologiche per il miglioramento di processi produttivi di principi attivi farmaceutici di interesse industriale e ricerca di nuove molecole bioattive da sorgenti naturali
Capofila: Sanofi-Aventis S.p.A.

PON01_02782 Nuove strategie nanotecnologiche per la messa a punto di farmaci e presidi diagnostici diretti verso cellule cancerose circolanti (CTC).
Capofila: Biogem S.c.a r.l.

RESIGRASS - An holistic understanding of seagrass functioning and resilience to local-scale disturbances: from molecular to biogeographical scales

S&T MED - finanziato dal Programma Operativo ENPI CBC Mediterranean Sea Basin 2007/2013 dell’Unione Europea

2019

Albarano L., Ruocco N., Ianora A., Libralato G., Manfra L. and Costantini M. (2019). Molecular and Morphological Toxicity of Diatom-Derived Hydroxyacid Mixtures to Sea Urchin Paracentrotus lividus Embryos. Marine Drugs, 17(3): 144.

Balzano S, Villanueva L, de Bar M, Sahonero Canavesi DX, Yildiz C, Engelmann JC, Marechal E, Lupette J, Sinninghe Damsté JS, Schouten S (2019) Biosynthesis of Long Chain Alkyl Diols and Long Chain Alkenols in Nannochloropsis spp. (Eustigmatophyceae). Plant and Cell Physiology, pcz078, https://doi.org/10.1093/pcp/pcz078

Di Costanzo F, Di Dato V, Ianora A, Romano G (2019) Prostaglandins in Marine Organisms: A Review. Marine Drugs 17: 428. https://doi.org/10.3390/md17070428

Di Dato V, Di Costanzo F, Barbarinaldi R, Perna A, Ianora A, Romano G (2019) Unveiling the presence of biosynthetic pathways for bioactive compounds in the Thalassiosira rotula transcriptome. Scientific Reports 9: 9893. https://doi.org/10.1038/s41598-019-46276-8

Galasso C, D’Aniello S, Sansone C, Ianora A, Romano G (2019) Identification of Cell Death Genes in Sea Urchin Paracentrotus lividus and Their Expression Patterns during Embryonic Development. Genome Biology and Evolution 11: 586-596. https://doi.org/10.1093/gbe/evz020

Galasso C, Gentile A, Orefice I, Ianora A, Bruno A, Noonan DM, Sansone C, Albini A, Brunet C (2019) Microalgal Derivatives as Potential Nutraceutical and Food Supplements for Human Health: A Focus on Cancer Prevention and Interception. Nutrients 11: 1226. https://doi.org/10.3390/nu11061226

Graziani V, Esposito A, Scognamiglio M, Chambery A, Russo R, Ciardiello F, Troiani T, Potenza N, Fiorentino A, D’Abrosca B (2019) Spectroscopic Characterization and Cytotoxicity Assessment towards Human Colon Cancer Cell Lines of Acylated Cycloartane Glycosides from Astragalus boeticus L. Molecules 24: 1725. Available: http://dx.doi.org/10.3390/molecules24091725

Graziani V, Scognamiglio M, Esposito A, Fiorentino A, D’Abrosca B (2019) Chemical diversity and biological activities of the saponins isolated from Astragalus genus: focus on Astragaloside IV. Phytochemistry Reviews. https://doi.org/10.1007/s11101-019-09626-y

Izabela K-M, Monika B, Vanja T, Adriana A, Clementina S, Pierre A, Mustapha C-M, Gérard L, Douglas N, Sladjana S, Agnieszka B (2019) Prophylaxis of Non-communicable Diseases: Why Fruits and Vegetables may be Better Chemopreventive Agents than Dietary Supplements Based on Isolated Phytochemicals? Current Pharmaceutical Design 25: 1-14.  DOI: 10.2174/1381612825666190702093301

Lauritano, C.; Ferrante, M.I.; Rogato, A. (2019) Marine Natural Products from Microalgae: An -Omics Overview. Mar. Drugs, 17, 269. https://doi.org/10.3390/md17050269

Martínez KA, Lauritano C, Druka D, Romano G, Grohmann T, Jaspars M, Martín J, Díaz C, Cautain B, de la Cruz M, Ianora A, Reyes F (2019) Amphidinol 22, a New Cytotoxic and Antifungal Amphidinol from the Dinoflagellate Amphidinium carterae. Marine Drugs 17: 385. https://doi.org/10.3390/md17070385

Merquiol L, Romano G, Ianora A, D’Ambra I. (2019) Biotechnological Applications of Scyphomedusae. Marine Drugs. 17(11):604. https://doi.org/10.3390/md17110604

Morroni L, Sartori D, Costantini M, Genovesi L, Magliocco T, Ruocco N, Buttino I (2019) First molecular evidence of the toxicogenetic effects of copper on sea urchin Paracentrotus lividus embryo development. Water Research 160: 415-423 https://doi.org/10.1016/j.watres.2019.05.062

Nuzzo G., Gallo C., D’Ippolito G., Manzo E., Ruocco N., Russo E., Carotenuto Y., Costantini M., Zupo V., Sardo A. and Fontana A. (2019). UPLC–MS/MS Identification of Sterol Sulfates in Marine Diatoms. Mar. Drugs, 17(1), 10; https://doi.org/10.3390/md17010010

Nuzzo G, Gomes BA, Gallo C, Amodeo P, Sansone C, Pessoa ODL, Manzo E, Vitale RM, Ianora A, Santos EA, Costa-Lotufo LV, Fontana A. (2019) Potent Cytotoxic Analogs of Amphidinolides from the Atlantic Octocoral Stragulum bicolor. Mar Drugs. 2019 Jan 16;17(1). doi: 10.3390/md17010058

Ruocco N, Annunziata C, Ianora A, Libralato G, Manfra L, Costantini S, Costantini M (2019) Toxicity of diatom-derived polyunsaturated aldehyde mixtures on sea urchin Paracentrotus lividus development. Scientific Reports 9: 517. https://doi.org/10.1038/s41598-018-37546-y

Ruocco N, Cavaccini V, Caramiello D, Ianora A, Fontana A, Zupo V, Costantini M (2019) Noxious effects of the benthic diatoms Cocconeis scutellum and Diploneis sp. on sea urchin development: Morphological and de novo transcriptomic analysis. Harmful Algae 86: 64-73. https://doi.org/10.1016/j.hal.2019.05.009

Russo C, Graziani V, Lavorgna M, D’Abrosca B, Piscitelli C, Fiorentino A, Scognamiglio M, Isidori M (2019) Lymphocytes exposed to vegetables grown in waters contaminated by anticancer drugs: metabolome alterations and genotoxic risks for human health. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 842: 125-131.

Russo E, d’Ippolito G, Fontana A, Sarno D, D’Alelio D, Busseni G, Ianora A, von Elert E, Carotenuto Y (2019) Density-dependent oxylipin production in natural diatom communities: possible implications for plankton dynamics. The Isme Journalhttps://doi.org/10.1038/s41396-019-0518-5

Sansone C, Brunet C (2019) Promises and Challenges of Microalgal Antioxidant Production. Antioxidants 8: 199. https://doi.org/10.3390/antiox8070199

Scognamiglio M, Graziani V, Tsafantakis N, Esposito A, Fiorentino A, D'Abrosca B (2019) NMR-based metabolomics and bioassays to study phytotoxic extracts and putative phytotoxins from Mediterranean plant species. Phytochemical Analysis 30: 512-523. https://doi.org/10.1002/pca.2842

Smerilli A, Balzano S, Maselli M, Blasio M, Orefice I, Galasso C, Sansone C, Brunet C. (2019) Antioxidant and Photoprotection Networking in the Coastal Diatom Skeletonema marinoi. Antioxidants, 8(6):154.  
https://doi.org/10.3390/antiox8060154

Vingiani GM, De Luca P, Ianora A, Dobson AD, Lauritano C. (2019) Microalgal Enzymes with Biotechnological Applications. Marine Drugs. 17(8):459. https://doi.org/10.3390/md17080459

Zupo V. (2019) Co-evolution of the Shrimp Hippolyte inermis and the Diatoms Cocconeis spp. in Posidonia oceanica: Sexual Adaptations Explained by Ecological Fitting. In: Merillon JM., Ramawat K. (eds) Co-Evolution of Secondary Metabolites. Reference Series in Phytochemistry. Springer, Cham

Zupo V., Mutalipassi M., Ruocco N., Glaviano F., Pollio A., Langellotti A.L., Romano G. and Costantini M. (2019) Distribution of Toxigenic Halomicronema spp. in Adjacent Environments on the Island of Ischia: Comparison of Strains from Thermal Waters and Free Living in Posidonia Oceanica Meadows. Toxins, 8;11(2).

2018

Galasso C., Orefice I., Pellone P., Cirino P., Miele R., Ianora A., Brunet C., and Sansone C. (2018) On the Neuroprotective Role of Astaxanthin: New Perspectives? Marine Drugs, 16, 247; doi:10.3390/md16080247

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2016

Addamo, A. M., Vertino, A., Stolarski, J., García-Jiménez, R., Taviani, M. and Machordom, A. (2016). Merging scleractinian genera: the overwhelming genetic similarity between solitary Desmophyllum and colonial Lophelia. BMC evolutionary biology 16.

Amaro, T., Huvenne, V., Allcock, A., Aslam, T., Davies, J., Danovaro, R., De Stigter, H., Duineveld, G., Gambi, C. and Gooday, A. (2016). The Whittard Canyon–A case study of submarine canyon processes. Progress in Oceanography 146, 38-57.

Amato, A., Fortini, S., Watteaux, R., Diano, M., Espa, S., Esposito, S., Ferrante, M. I., Peters, F., Iudicone, D. and d'Alcala, M. R. (2016). TURBOGEN: Computer-controlled vertically oscillating grid system for small-scale turbulence studies on plankton. Review of Scientific Instruments 87.

Balzano, S., Percopo, I., Siano, R., Gourvil, P., Chanoine, M., Marie, D., Vaulot, D. and Sarno, D. (2016) Morphological and genetic diversity of Beaufort Sea diatoms with high contributions from the Chaetoceros neogracilis species complex. Journal of Phycology, DOI: 10.1111/jpy.12489

Bastianini, M., Totti, C., Penna, A., De Lazzari, A. & Montresor, M. (2016). Dinoflagellate cyst production in the north-western Adriatic Sea. Medit. Mar. Sci.:DOI: http://dx.doi.org/10.12681/mms.1770.

Bertocci, I., Arenas, F., Cacabelos, E., Martins, G. M., Seabra, M. I., Álvaro, N. V., Fernandes, J. N., Gaião, R., Mamede, N., Mulas, M., Neto, A.I. (2016). Nowhere safe? Exploring the influence of urbanization across mainland and insular seashores in continental Portugal and the Azorean Archipelago. Marine Pollution Bulletin 114, 644–655.

Bonanno, A., Barra, M., Basilone, G., Genovese, S., Rumolo, P., Goncharov, S., Popov, S., Buongiorno Nardelli, B., Iudicone, D. and Procaccini, G. (2016). Environmental processes driving anchovy and sardine distribution in a highly variable environment: the role of the coastal structure and riverine input. Fisheries Oceanography 25, 471-490.

Brugnano, C., Granata, A., Guglielmo, L., Minutoli, R., Zagami, G. and Ianora, A. (2016). The deleterious effect of diatoms on the biomass and growth of early stages of their copepod grazers. Journal of Experimental Marine Biology and Ecology 476, 41-49.

Bulleri F, Badalamenti F, Iveša L, Mikac B, Musco L, Jaklin A, Rattray AJ, Vega Fernández T, Benedetti-Cecchi L (2016). The effects of an invasive seaweed on native communities vary along a gradient of land-based human impacts. Peer J 4:e1795 (https://doi.org/10.7717/peerj.1795).

Buttino, I., Hwang, J.-S., Romano, G., Sun, C.-K., Liu, T.-M., Pellegrini, D., Gaion, A. and Sartori, D. (2016). Detection of malformations in sea urchin plutei exposed to mercuric chloride using different fluorescent techniques. Ecotoxicology and Environmental Safety 123, 72-80.

Castellani, C., Licandro, P., Fileman, E., Di Capua, I. and Mazzocchi, M. G. (2016). Oithona similis likes it cool: evidence from two long-term time series. Journal of Plankton Research 38, 703-717.

Catanese, G., Montes, I., Iriondo, M., Estonba, A., Iudicone, D. and Procaccini, G. (2016). High resolution SNPs selection in Engraulis encrasicolus through Taqman OpenArray. Fisheries Research 177, 31-38.

Chust, G., Villarino, E., Chenuil, A., Irigoien, X., Bizsel, N., Bode, A., Broms, C., Claus, S., Fernandez de Puelles, M. L., Fonda-Umani, S., Hoarau, G., Mazzocchi, M.G., Mozetič, P., Vandepitte, L., Veríssimo, E., Zervoudaki, S., Borja, A. (2016). Dispersal similarly shapes both population genetics and community patterns in the marine realm. Scientific Reports 6.

Corriero, G., Pierri, C., Accoroni, S., Alabiso, G., Bavestrello, G., Barbone, E., Bastianini, M., Bazzoni, A. M., Aubry, F. B., Boero, F. Buia, M.C., Cabrini, M., Camatti, E., Cardone, F., Cataletto, B., Cattaneo Vietti, R., Cecere, E., Cibic, T., Colangelo, P., De Olazabal, A., D'Onghia, G., Finotto, S., Fiore, N., Fornasaro, D., Fraschetti, S., Gambi, M.C., Giangrande, A., Gravili, C., Guglielmo, R., Longo, C., Lorenti, M., Lugliè, A., Maiorano, P., Mazzocchi, M.G., Mercurio, M., Mastrototaro, F., Mistri, M., Monti, M., Munari, C., Musco, L., Nonnis Marzano, C., Padedda, B.C., Patti, F.P., Petrocelli, A., Piraino, S., Portacci, G., Pugnetti, A., Pulina, S., Romagnoli, T., Rosati, I., Sarno, D., Satta, C.T., Sechi, N., Schiaparelli, S., Scipione, B., Sion, L., Terlizzi, A., Tirelli, V., Totti, C., Tursi, A., Ungaro, N., Zingone, A., Zupo, V., Basset, A. (2016). Ecosystem vulnerability to alien and invasive species: a case study on marine habitats along the Italian coast. Aquatic Conservation-Marine and Freshwater Ecosystems 26, 392-409.

D’Alelio, D., Libralato, S., Wyatt, T. and d’Alcalà, M. R. (2016). Ecological-network models link diversity, structure and function in the plankton food-web. Scientific reports 6.

D'Alelio, D., Montresor, M., Mazzocchi, M. G., Margiotta, F., Sarno, D. and d'Alcalà, M. R. (2016). Plankton food-webs: to what extent can they be simplified? Advances in Oceanography and Limnology 7.

De Luca D., Catanese G., Procaccini G., Fiorito G. (2016). Octopus vulgaris in the Mediterranean sea: genetic diversity and population structure. Plos One 11(2): e0149496. doi:10.1371/journal.pone.0149496

D’Esposito D., Orrù L., Dattolo E., Bernardo L., Lamontara A., Orsini L., Serra I.A.,  Mazzuca S., Procaccini G. (2016). Annotation of "de novo" transcriptome in the seagrass Posidonia oceanica and SSR markers development. Scientific Data 3:160115 doi: 10.1038/sdata.2016.115.

Di Lorenzo M, Vega Fernández T, Badalamenti F, Guidetti P, Starr RM, Giacalone VM, Di Franco A, D’Anna G (2016). Diel activity and variability in habitat use of white sea bream in a temperate Marine Protected Area. Marine Environmental Research 116:1-9 (http://dx.doi.org/10.1016/j.marenvres.2016.02.007).

Farrant, G. K., Dore, H., Cornejo-Castillo, F. M., Partensky, F., Ratin, M., Ostrowski, M., Pitt, F. D., Wincker, P., Scanlan, D. J., Iudicone, D., Acinasb, S.G., Garczareka, L. (2016). Delineating ecologically significant taxonomic units from global patterns of marine picocyanobacteria. Proceedings of the National Academy of Sciences of the United States of America 113, E3365-E3374.

Forster, D., Dunthorn, M., Mahé, F., Dolan, J. R., Audic, S., Bass, D., Bittner, L., Boutte, C., Christen, R. and Claverie, J.-M. (2016). Benthic protists: the under-charted majority. FEMS microbiology ecology, fiw120.

Fortunato, A. E., Jaubert, M., Enomoto, G., Bouly, J.-P., Raniello, R., Thaler, M., Malviya, S., Bernardes, J. S., Rappaport, F. and Gentili, B. (2016). Diatom phytochromes reveal the existence of far-red light based sensing in the ocean. The Plant Cell, tpc. 00928.2015.

Gallina, A. A., Palumbo, A. and Casotti, R. (2016). Oxidative pathways in response to polyunsaturated aldehydes in the marine diatom Skeletonema marinoi (Bacillariophyceae). Journal of phycology.

Gambi MC, Patti FP (2016). Ritrovamento di “perle” in un esemplare del mollusco bivalve Crassostrea gigas (Thurnberg) (Ostreidae, Bivalvia) a Salerno. Notiziario S.I.B.M. 69: 44-48.

Gambi MC, Lorenti M, Patti FP, Zupo V (2016). An annotated list of alien marine species of the Ischia Island (Gulf of Naples). Notiziario S.I.B.M. 70: 64-68.

Gambi MC, Musco L, Giangrande A, Badalamenti F, Micheli F, Kroeker KJ. (2016). Distribution and functional traits of polychaetes in a CO2 vent system: winners and losers among closely related species. Marine Ecology Progress Series, 550:121-13.

Gara, A.B., R.B.A. Kolsi, R. Chaaben, N. Hammami, M. Kammoun, F.P. Patti, A. El Feki, L. Fki, H. Belghith and K. Belghith (2016). Inhibition of key digestive enzymes related to hyperlipidemia and protection of liver-kidney functions by Cystoseira crinita sulphated polysaccharide in high-fat diet-fed rats. Biomedicine & Pharmacotherapy 85: 517-526. DOI: 10.1016/j.biopha.2016.11.059.

Garrido, J. L., Brunet, C. and Rodríguez, F. (2016). Pigment variations in Emiliania huxleyi (CCMP370) as a response to changes in light intensity or quality. Environmental microbiology 18: 4412–4425. doi:10.1111/1462-2920.13373.

Gherardi, M., Amato, A., Bouly, J.-P., Cheminant, S., Ferrante, M. I., d'Alcalá, M. R., Iudicone, D., Falciatore, A. and Lagomarsino, M. C. (2016). Regulation of chain length in two diatoms as a growth-fragmentation process. Physical Review E 94, 022418.

Guidi, L., Chaffron, S., Bittner, L., Eveillard, D., Larhlimi, A., Roux, S., Darzi, Y., Audic, S., Berline, L., Brum, J. R., Coelho, L.P., Espinoza, J.C., Malviya, S., Sunagawa, S., Dimier, C., Kandels-Lewis, S., Picheral, M., Poulain, J., Searson, S.; Tara Oceans Consortium Coordinators, Stemmann, L., Not, F., Hingamp, P., Speich, S., Follows, M., Karp-Boss, L., Boss, E., Ogata, H., Pesant, S., Weissenbach, J., Wincker, P., Acinas, S.G., Bork, P., de Vargas, C., Iudicone, D., Sullivan, M.B., Raes, J., Karsenti, E., Bowler, C., Gorsky, G. (2016). Plankton networks driving carbon export in the oligotrophic ocean. Nature 532, 465-+.

Iudicone, D., Rodgers, K. B., Plancherel, Y., Aumont, O., Ito, T., Key, R. M., Madec, G. and Ishii, M. (2016). The formation of the ocean’s anthropogenic carbon reservoir. Scientific reports 6.

Jahnke, M., Christensen, A., Micu, D., Milchakova, N., Sezgin, M., Todorova, V., Strungaru, S. and Procaccini, G. (2016). Patterns and mechanisms of dispersal in a keystone seagrass species. Marine Environmental Research 117, 54-62.

Jaspars, M., De Pascale, D., Andersen, J. H., Reyes, F., Crawford, A. D. and Ianora, A. (2016). The marine biodiscovery pipeline and ocean medicines of tomorrow. Journal of the Marine Biological Association of the United Kingdom 96, 151-158.

Kamenos N., Perna G., Gambi, M.C., Micheli F., Kroeker K. (2016) Coralline algae in a naturally acidified ecosystem persist by maintaining control of skeletal mineralogy and size. Proceedings Royal Society B (Biological Sciences), 283: 20161159. dx.doi.org/10.1098/rspb.2016.1159.

Kolsi, R. B. A., Fakhfakh, J., Krichen, F., Jribi, I., Chiarore, A., Patti, F. P., Blecker, C., Allouche, N., Belghith, H. and Belghith, K. (2016). Structural characterization and functional properties of antihypertensive Cymodocea nodosa sulfated polysaccharide. Carbohydrate Polymers.

Lauritano, C., Andersen, J. H., Hansen, E., Albrigtsen, M., Escalera, L., Esposito, F., Helland, K., Hanssen, K. Ø., Romano, G. and Ianora, A. (2016a). Bioactivity screening of microalgae for antioxidant, anti-inflammatory, anticancer, anti-diabetes and antibacterial activities. Frontiers in Marine Science 3, 68.

Lauritano C, Andersen JH, Hansen E, Albrigtsen M, Escalera L, Esposito F, Helland K, Hansen K Ø, Romano G, Ianora A. (2016). The potential of drug discovery from marine microalgae. Frontiers in Marine Biotechnology, May 2016, 3, 68.

Lauritano C and Ianora A. (2016). Marine Organisms with Anti-diabetes properties. Mar. Drugs, 14, 220; doi:10.3390/md14120220.

Lauritano, C., Romano, G., Roncalli, V., Amoresano, A., Fontanarosa, C., Bastianini, M., Braga, F., Carotenuto, Y. and Ianora, A. (2016b). New oxylipins produced at the end of a diatom bloom and their effects on copepod reproductive success and gene expression levels. Harmful Algae 55, 221-229.

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