Basic Services for Molecular Biology
Preparation and distribution of media and plates for bacteriology, of media for animal cell culture, of several products and reagents for Molecular Biology and ISH, of competent cells for bacterial transformation etc. Provisioning of synthetic oligonucleotides.
Qualitative control of nucleic acids
Qualitative and quantitative analysis of nucleic acids and definition of the reference standard for the QC of the RNA samples by microfluidic chip.
Real Time qPCR
Real Time qPCR mainly used for analysis of gene expression. Support to users includes assistance with experimental design, running of samples, and if requested, initial data analysis.
Droplet Digital PCR (in progress)
The ddPCR allows to:
Detect small amounts of target DNA molecules, with high sensitivity.
Determine the change in the number of genomic copies with high accuracy.
Measure gene expression levels with high precision.
Quantify NGS libraries.
DNA Sequencing service
Automated DNA sequencing by capillary electrophoresis essential for the study of nucleic acids from plasmids, PCR fragments , phage , etc.
Genotyping service
Used for genetic population studies by analysis of::
- Microsatelliti (single-locus).
- Snips, (single-locus).
- AFLP, (multi-locus).
Automation and High Throughput Screening (HTS) projects
Realization in automation, by Robotics (liquid handler), of a high-throughput projects for those research activities that require, in the short term, the analysis of a large number of samples.
Next Generation Sequencing service (NGS) (in progress)
Next generation sequencing (NGS) using "ION Proton" (Life Technologies) can produce data for transcriptomic studies, metagenomics, gene expression analysis, and targeted Resequencing.
