NEW Program
The Electron Microscopy Hands-On Course: sample preparation and imaging of marine environmental samples arises from a very strong and active collaboration between the Stazione Zoologica Anton Dohrn and the EMBL in Heidelberg.
The aim of the course is to provide a theoretical and practical training on electron microscopy starting from the basics to the preparation of samples with high pressure freezing and image acquisition using TEM and SEM.
The course will also include lectures on advanced and recently developed technologies as volume electron microscopy and correlative light-electron microscopy.
The course will be held at the Stazione Zoologica Anton Dohrn in Naples on 6-8 November 2023 and will target in particular researchers and technicians from scientific institutions interested in the field of electron microscopy. No documented experience in electron microscopy is required.
Theoretical lessons
Sample preparation for EM – Chemical fixations
Sample preparation for EM - High pressure Freezing- Freeze substitution
Image formation in TEM and SEM
Use of SEM and TEM in Taxonomy of Marine Organisms
Practical lessons
Sample preparation for SEM: sample sorting, fixation, dehydration, critical point drying, sputtering
High pressure Freezing
Ultramicrotomy
Observation at TEM
Observation at SEM
Lectures
Immunolabeling
Correlative Light Electron Microscopy
Volume Electron Microscopy
Teachers
Giovanna Benvenuto, SZN
Fabio Formiggini, IIT Napoli, SZN
Frédéric Leroux, Leica Microsystems
Paolo Ronchi, EMBL, Heidelberg
Diana Sarno, SZN
Lecturers
Raman Parashuraman, Euro-Bioimaging, Napoli
Roman Polishchuck, TIGEM, Napoli, Italy
Paolo Ronchi, EMBL, Heidelberg
Luigia Santella, SZN
Keynote lecturer
Detlev Arendt, EMBL, Heidelberg
Teaching assistants
Filomena Caccavale, Maria Cocurullo, Rita Graziano, Franco Iamunno, Isabella Percopo, Lorenza Rusciano, Eleonora Scalco (SZN), Marinella Pirozzi (CNR)
IMPORTANT INFORMATION
Application deadline September, 8th
Accepted participants will be notified by September, 15th
Registration and payment are due by September, 30th
Fee: 200 euro per person (coffee breaks, lunches, and social dinner included)
The course will be open to 14 participants. Preference will be given to strongly motivated applicants.
ORGANIZERS: Giovanna Benvenuto and Paolo Ronchi
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